TelN Protelomerase (TLN-BE001)
Catalog: TLN-BE001
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Product Description | TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini. |
Synonyms | TelN; Phage N15 protelomerase; Protelomerase; TelN enzyme; TelN protein; |
Application | In vitro enzymatic synthesis of DNA constructs |
Concentration | 10U/µL |
Unit Definition | One unit is defined as the amount of enzyme required to cut 0.5 μg completely BsaI linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) at 30°C for 30 min in a total reaction volume of 20 μL. |
Source | E.coli strain that carries TelN from phage N15 |
Form | Liquid |
Shipping | Shipped with dry ice |
Stability And Storage | -20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
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