Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)

  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr21-Ile192

Catalog: CD2-HM227B

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Product Description Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Thr21-Ile192.

Product Background

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Product Category

Recombinant Protein

Target

CD27/TNFRSF7

Gene/Protein ID

P26842

Species

Human

Exact Sequence

Thr21-Ile192
Molecular Weight The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA.
The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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